Mannitol salt agar

Mannitol salt agar growth media used in the laboratory. Do you know how to make the medium of this bacteria?
Perhaps you have heard of the growth medium or culture media used in the laboratory of different types. This media can be liquid or gel type and cell culture and microbial culture. In other words, the growth media for growing cells (from plants or animals) or micro-organisms, like bacteria, yeast, etc., are rich in nutrients to help the growth of these microorganisms in large colonies, the bacterial culture. Composition of the chemicals required in the amount of water, nutrients, etc. Although these cultures, these media some simple liquid or solid form, peptone water, agar, etc., there are some nutrient-rich blood agar, chocolate agar, mannitol salt agar (MSA), a growth medium, which means that the bacteria.
What is mannitol salt agar
The composition of growth medium of certain nutrients, agar powder, sodium chloride, phenol red, To be more precise, this growing medium made from casein (5 g per liter), agar (15 g per liter), D mannitol (10 g) per liter of enzyme digestion, phenol red (0.025 grams per liter), sodium chloride (75 g per liter) in animal tissues by enzyme digestion (5 g per liter), beef extract (1 gram per liters). This mixture of pH, temperature of 25 degrees Celsius is 7.4.
Mannitol salt agar selectivity and worse. It is selective, because this medium contains the amount of salt, thus inhibiting the growth of almost all bacteria, is the gold except. Therefore, it is used for isolation of Staphylococcus bacteria. This medium is also bad, because it helps determine the different species of Staphylococcus bacteria in.
Mannitol salt agar test work
Because it has a high salt content, only Staphylococcus aureus bacteria was found to grow in this medium. As mentioned above, mannitol salt agar, because it helps determine the different types of staph bacteria. This is to help the phenol red (dye), change the color by certain bacteria release organic waste fermentation of mannitol. In case of Staphylococcus aureus, non-pathogenic species are not found, the color changes of fermented mannitol and media. However, the pathogenic Staphylococcus aureus species, produced by fermentation of organic waste such as Staphylococcus aureus mannitol and change in the bright yellow color. Staphylococcus epidermidis, usually this change does not occur in the case found in human skin color. In the case of coagulase-positive staphylococci (ie, the coagulation of blood), mannitol salt agar results show that the yellow color change. Coagulase-negative staphylococci, even if it is found in the body’s normal flora, these bacteria may cause serious infection in immunocompromised populations. The bacterial growth of small pink or red colonies, without changing the color of the medium. The coagulation enzyme-positive bacteria were found to grow into a large colony of yellow, changing the medium to yellow color.
This is only about a mannitol salt agar and working mechanism of a brief overview. Under normal circumstances, it is said, the typical Staphylococcus aureus pathogenicity of this medium, fermentation, become yellow, and vice versa, non-pathogenic form of red or pink colonies.
OTHER ARTICLE: Cassia Gum – Natural Polymer & How to use Food Thickeners